This toolkit lays important groundwork to help teachers successfully enact motivation design principles (MDPs) in support of student motivation and engagement in science. It addresses the reciprocal relationship between motivationally supportive teaching and equitable science instruction and provides guidance on general classroom organization, routines, policies, and climate considerations that provide a vital foundation for supporting student motivation. The toolkit contains resources for the five MDPs: belonging, confidence, learning orientation, autonomy, and relevance. It describes connections between enacting the MDPs and delivering instruction based on the NGSS and outlinines the reciprocal relationship between supporting student motivation and implementing phenomenon-centered science instruction, integrating crosscutting concepts, and promoting the eight science and engineering practices from the NGSS.